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Valtieri et al., J Immunol, 138: 21 (1987) [PMID: 2438328] Migliaccio et al., J Cell Biol, 109: 21 (1989) [PMID: 2668305] CABRI:DSMZ_MUTZ Accession_number ACC 411 Cell_line_name 32D Brief_description confirmed as mouse with IEF of LDH, NP established from long-term bone marrow cultures of C3H/HeJ mice infected with the Friend murine leukemia virus; cells are constitutively growth factor-dependent Description mouse bone marrow established from long-term bone marrow cultures of C3H/HeJ mice infected with the Friend murine leukemia virus; cells are constitutively growth factor-dependent confirmed as mouse with IEF of LDH, NP Depositor Dr. H. G. Drexler, DSMZ, Braunschweig, Germany Morphology mostly round cells growing singly or in small clusters in suspension, some cells are loosely adherent Culture_conditions 90% RPMI 1640 + 10% FBS + 10-20% vol conditioned medium of cell line WEHI-3B (DSM ACC 26) maintain at 0.02-1.5 x 106 cells/ml; split 1:10 to 1:50 every 2-3 days; seed out at ca. 0.5 x 106 cells/ml. CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and cell density. Thus the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines. at 37 C with 5% CO2 maximum density at ca. 1.5 x 106 cells/ml Viruses ELISA: reverse transcriptase negative; PCR: SMRV - Properties Release_conditions - Hazard - Storage frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule Doubling_time ca. 15 hours Mycoplasma negative in DAPI, microbiological culture, RNA hybridization, PCR assays Cytogenetics murine hypodiploid karyotype - 35(34-35)<2n> - carries 5 centric fusion markers Bibliographic_reference Greenberger et al., Proc Natl Acad Sci USA, 80: 21 [PMID: 6574462 ]

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